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  7. Thermo Scientific™ Phire Tissue Direct PCR Master Mix
  8. 15252606

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Brand:  Thermo Scientific™ F170L

4933.58 DKK valid until 2025-03-21
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Product Code. 15252606

  • 7400.00 DKK / 500 reactions

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Description

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• DNARelease Additive, 1.3 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• 50 μg O'GeneRuler Express DNA Ladder

Store at -20°C.
Direct PCR
Fast
Direct PCR Master Mix
Wet or Dry Ice
2X
1250 Reactions
SuperMix or Master Mix
DNA
Medium
Phire Hot Start II DNA Polymerase
Yes
1,250 reactions
Genotyping, Hot-start PCR
Built-In Hot Start
Blunt
Tissue
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2874307 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog NumberF170L
Lot Number2813400 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog NumberF170L
Lot Number2912581 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog NumberF170L
Lot Number3112209 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog NumberF170L
Lot Number2968420 Certificate TypeCertificate of Analysis Date12/02/2025 Catalog NumberF170L
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