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Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Brand: Thermo Scientific™ F170S

1101.38 DKK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

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Product Code. 15264756

1652.00 DKK / 250 reactions

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

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Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

Direct PCR reduces time-consuming and expensive DNA purification steps
Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
Specially engineered Phire DNA polymerase for extremely short PCR protocol times
Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

Genotyping
Transgene detection
Knockout analysis
Sequencing

Notes

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

Content And Storage	• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL • Dilution Buffer, 5 mL • DNARelease Additive, 3 x 100 μL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 2 x 1.25 mL • 50 μg O'GeneRuler Express DNA Ladder Store at -20°C.
PCR Method	Direct PCR
Reaction Speed	Fast
Product Type	Direct PCR Master Mix
Shipping Condition	Wet or Dry Ice
Concentration	2X
No. of Reactions	250 Reactions
Reaction Format	SuperMix or Master Mix
Starting Material	DNA

GC-Rich PCR Performance	Medium
Polymerase	Phire Hot Start II DNA Polymerase
Includes Label or Dye	Yes
Quantity	250 reactions
For Use With (Application)	Genotyping, Hot-start PCR
Hot Start	Built-In Hot Start
Overhang	Blunt
Sample Type	Tissue

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

Phire Tissue Direct PCR Master Mix/DNARelease Additive

Signal Word

Danger

Hazard Category

Respiratory sensitiser Category 1

Hazard Statement

H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P284-¢In case of inadequate ventilation! wear respiratory protection.
P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P501-Dispose of contents/container to .

Supplemental information

MIXTURE LIST-Contains : Proteinase, tritirachium album serine

Documents

Scientific Resources

Brochures

Phire Tissue Direct PCR Master Mix and Kit—species and sample types tested File size: 34.7 kB

Phire Tissue Direct PCR Master Mix and Kit—sample size recommendations File size: 42.5 kB

Phire Animal Tissue Direct PCR Master Mix—recommended control primer sequences for Drosophila and zebrafish File size: 411.9 kB

Reference Materials

LabAid: Direct PCR solutions File size: 65.2 kB

White Paper: Animal-friendly mouse genotyping using direct PCR File size: 378.2 kB

Application Notes

Brochure: Thermo Scientific Direct PCR—amplify without purification File size: 1.9 MB

Product Information

Manuals

Product Information: Thermo Scientific Phire Tissue Direct PCR Master Mix File size: 160 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3119678	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberF170S
Lot Number3151945	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberF170S
Lot Number3144107	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberF170S
Lot Number3131875	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberF170S
Lot Number3151943	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberF170S

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Thermo Scientific™ Phire Tissue Direct PCR Master Mix > 250 Reactions

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