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Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

1590.00 DKK - 7120.00 DKK

Specifications

GC-Rich PCR Performance Medium
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Speed Fast
Includes Label or Dye Yes
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
15264756
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Thermo Scientific™
F170S
250 Reactions
1590.00 DKK
250 reactions
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15252606
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Thermo Scientific™
F170L
1250 Reactions
7120.00 DKK
500 reactions
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Description

Description

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Features

  • Direct PCR reduces time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
  • Specially engineered Phire DNA polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications

Specifications

Medium
Phire Hot Start II DNA Polymerase
Yes
Wet or Dry Ice
2X
Blunt
Tissue
Direct PCR
Fast
Direct PCR Master Mix
Genotyping, Hot-start PCR
Built-In Hot Start
SuperMix or Master Mix
DNA
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Phire Tissue Direct PCR Master Mix/DNARelease Additive

Signal Word
  • Danger

Hazard Category
  • Respiratory sensitiser Category 1

Hazard Statement
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : Proteinase, tritirachium album serine

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.