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Substrates

Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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1 – 15 of 275 results
1

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate

Deubiquitinating enzymes (DUBs) play housekeeping functions by maintaining pools of active ubiquitin, and regulatory function by rescuing specific target proteins from degradation by the proteasome.

3

Thermo Scientific™ GFP-4E-BP1

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).

4

Thermo Scientific™ CHKtide

A peptide substrate based upon CDC25C that is phosphorylated by CHEK1 and other kinases.

5

Thermo Scientific™ CDK7/9tide Substrate

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).

6

Invitrogen™ PI(3,4)P2 Lipid Kinase Substrate

The PI(3,4)P2 Lipid Kinase Substrate has been specifically optimized for use with Type I PIP kinases (e.g., PIP5K1A, PIP5K1B, PIP5K1C)

Shipping Condition Dry Ice
Substrate Phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2)
Content And Storage Phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) in 30 mM HEPES pH 7.5 buffer.
Form Powder
Substrate Type Kinase Substrate
Product Line Invitrogen™
7

Thermo Scientific™ Vivid™ BOMR Substrate

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs.

Shipping Condition Approved for shipment on Wet or Dry Ice
Substrate BOMR
Substrate Properties Chemical Substrate
Content And Storage Vivid™ CYP450 Screening kits include:
• Vivid™ CYP450 Fluorogenic substrates (also available separately)
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Substrate Type Cytochrome P450 Substrate
Product Line VIVID™
Detection Method Fluorescence
9

Molecular Probes™ CSPD™ Substrate (25 mM Concentrate)

This concentrated CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Shipping Condition Room Temperature
Substrate Alkaline Phosphatase
Content And Storage Store the product at 4°C.
Format Bottle(s)
Form Liquid
Substrate Type AP (Alkaline Phosphatase) Substrate
For Use With (Application) Southern, Northern, and Western Blotting
10

Thermo Scientific™ PI Lipid Kinase Substrate

Specifically optimized for use with the Class II (PIK3C2A & PIK3C2B) PI3 kinases.

14

Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Substrate Beta-Gal Substrate
Assay Reporter Enzyme
Substrate Properties Chemical Substrate
Content And Storage Store at -20°C.
Format 96-well Plate
Product Type Pierce beta-Galactosidase Assay Reagent
For Use With (Equipment) Microplate Reader
Form Liquid
Label or Dye Enzyme Labeled
Target Enzyme Beta-Galactosidase
For Use With (Application) Absorbance
Product Line Pierce™
Detection Method Colorimetric
15

Molecular Probes™ Galacton-Plus™ Substrate (100X Concentrate)

Galacton-Plus™ Substrate (100X Concentrate)

Shipping Condition Room Temperature
Substrate Beta-Galactosidase
Content And Storage Store the product at 4°C.
Format Bottle(s)
Form Liquid
Substrate Type Beta-Gal Substrate
Product Line Galacton-Plus™

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