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Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
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Form | Liquid |
Product Line | Restore™ |
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Invitrogen™ iBind™ Flex Solution Kit
Detection kit for use with iBind™ Flex Western System
No. of Reactions | 10 midi blots or 20 mini blots |
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Product Type | Flex Solution Kit |
For Use With (Equipment) | iBind Flex Western Device |
Membrane Compatibility | PVDF, Nitrocellulose |
Product Line | iBind™ |
Detection Method | Colorimetric, Chromogenic, Chemiluminescence |
Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
Shipping Condition | Dry Ice |
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Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
Product Type | Protein Labeling Reagent |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Membrane Compatibility | PVDF, Nitrocellulose |
Shelf Life | At least 2 years from receipt |
Run Time | 10 minutes |
For Use With (Application) | Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Product Line | Invitrogen™ |
Thermo Scientific™ Pierce™ 1-Step Transfer Buffer
Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.
Product Type | Transfer Buffer |
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Invitrogen™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ Bolt™ Antioxidant
A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)
Optimized, easy-to-use blocker and primary antibody diluent system
Invitrogen™ WesternBreeze™ Wash Solution (16X)
Optimized solution of concentrated buffered saline containing detergent
Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
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Form | Liquid |
Product Type | Western Blot Enhancer |
Membrane Compatibility | Nitrocellulose, PVDF |
For Use With (Application) | Western Blot |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Form | Liquid |
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Product Type | Western Blot Stripping Buffer |
Concentration | 10X |
Invitrogen™ Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675
Use with Novex Tricine Gels